PointSuppressor™Wild Type Suppression
SYNTHETIC NUCLEOTIDES AND METHODS FOR SELECTIVELY AMPLIFYING ALLELES
U.S. Provisional Patent Filed 02FEB2021.
SYNTHETIC NUCLEOTIDES AND METHODS FOR SELECTIVELY AMPLIFYING ALLELES
PointSuppressor™ – the new standard in rare allele detection. Next-Generation Sequencing (NGS) based liquid biopsy applications – whether to detect cancer, transplant rejection, fetal DNA, or antimicrobial resistance – require a tremendous number of sequence reads to find rare alleles. Most of these sequencing reads are uninformative, for instance, background wild type sequences in the case of mutation detection in cancer. PointSuppressor™ technology suppresses these uninformative reads without interfering with amplification of the desired targets. The assay footprint is kept short since no blockers are used. Accuracy is extremely high since no mutant sequences are ever introduced into the reaction.