Amplicon Enrichment for cell free DNA

Protected by US and European Patents

NexGenAbler™ is a single-tube Next Generation Sequencing (NGS) library prep method for quantitative mutation detection in circulating cell-free DNA. It incorporates a Dynamic Molecular Index™ and improves upon specificity and sensitivity of existing methods.

The NexGenAbler™ Amplicon Enrichment for cell free DNA

Technology Benefits

  • Single tube method preserves samples and maximizes sensitivity delivering industry leading limit of detection under 0. 1% mutant allele fraction (MAF) with 25 ng of DNA (typical amount in 20ml blood draw)
  • Labor efficient 2.5-hour workflow with < 30 minutes hands-on time that is simple to automate
  • Specifically designed for cell free DNA, efficient detection between 1ng to 50ng of DNA input
  • Excellent multiplexing scalability within a single multiplex PCR pool (1-100 amplicons)
  • Precise quantification of each detected mutant fragment
  • “Fragmentomics Ready” with elucidation of both 3’ and 5’ ends as well as fragment sizes
  • Detection of and analysis for single nucleotide variants (SNVs), small insertions and deletions (Indels), copy number variations (CNVs), gene fusions / splice variants

Proprietary Dynamic Molecular Indexing (DMI) enables industry leading efficiency and precise quantitation.

Each cell free DNA fragment is labelled with a random molecular index (barcode) without requiring end repair, adapters, or ligation. Random barcodes provide unparalleled diversity of molecular indexes: 4ˆ30 (~10ˆ18) to 4ˆ120 (~10ˆ72). No purification needed.

Protected by US and European Patents.