MethylMarker™
Methylation Detection

METHODS FOR IDENTIFYING MODIFIED BASES IN A POLYNUCLEOTIDE U.S.
Provisional Patent Filed 10JUN2020.

MethylMarker™ is a novel approach to detect DNA methylations that allow cancer without interfering with detection of the mutations that drive cancer.

MethylMarker™ enables a single, sample sparing work flow to measure genetic and epigenetic drivers of cancer which means more data points for cancer detection and lower costs. That’s something the current bisulfite Gold Standard just can’t do.

MethylMarker™
Methylation Detection

METHODS FOR IDENTIFYING MODIFIED BASES IN A POLYNUCLEOTIDE U.S.
Provisional Patent Filed 10JUN2020.

MethylMarker™ is a novel approach to detect DNA methylations that allow cancer without interfering with detection of the mutations that drive cancer.

MethylMarker™ enables a single, sample sparing work flow to measure genetic and epigenetic drivers of cancer which means more data points for cancer detection and lower costs. That’s something the current bisulfite Gold Standard just can’t do.
MethylMarker™ is a novel approach to detecting DNA methylation. It uses proprietary assay chemistry compatible with mutation detection to enable sample sparing, simultaneous measurement of genetic and epigenetic markers of cancer.

MethylMarker™ is a novel approach to detecting DNA methylation. It uses proprietary assay chemistry compatible with mutation detection to enable sample sparing, simultaneous measurement of genetic and epigenetic markers of cancer.

The Problem with the Gold Standard

Bisulfite-based approaches are the most-used methods to assess DNA methylation. Unfortunately, these destroy much of the DNA and convert all unmethylated cytosines (C) to thymine (T) signals. Newer enzymatic approaches are gentler, but suffer from similar issues. A C>T transition in one strand results in a G>A transition in the other strand. As a result, C>T and G>A mutations cannot be detected in these samples. Several prevalent mutations result from C>T or G>A transitions:
Table of common mutations where measurement is incompatible with existing assays that measure DNA methylation.

Currently, to measure methylation and mutation, DNA samples must be split into separate workflows. This doubles the number of samples to be analyzed and halves the amount of DNA available for each analysis.

The MethylMarker™ Solution

The MethylMarker™ Solution

MethylMarker™ leaves all unmodified C’s untouched and converts only the 5mC and 5hmC residues into T signals, the opposite of current methods. This simplifies assay design and sequence analysis and allows the entirety of a sample to be used for the simultaneous detection of both methylation and mutation states.

Advantages of MethylMarker™

  • Sample Sparing – more data points for a higher probability of determining the presence of absence of disease.
  • Single assay mutation and methylation analysis – both workload and costs are dramatically decreased.
  • Less destructive to DNA in samples – increased  probability of detecting rare events
Example of MethylMarker identification of methylated and mutated DNA.